Subtle variations in polymer chemistry modulates substrate stiffness and fibronectin activity

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dc.contributor.author	Brizuela Guerra, Nayrim	es_ES
dc.contributor.author	González-García, Cristina	es_ES
dc.contributor.author	Llopis, Virginia	es_ES
dc.contributor.author	Rodriguez-Hernandez, Jose Carlos	es_ES
dc.contributor.author	Moratal, David	es_ES
dc.contributor.author	Rico Tortosa, Patricia María	es_ES
dc.contributor.author	Salmerón Sánchez, Manuel	es_ES
dc.date.accessioned	2018-11-04T05:32:32Z
dc.date.available	2018-11-04T05:32:32Z
dc.date.issued	2010	es_ES
dc.identifier.issn	1744-683X	es_ES
dc.identifier.uri	http://hdl.handle.net/10251/111804
dc.description.abstract	[EN] A family of polymer substrates which consists of a vinyl backbone chain with the side groups ¿COO(CH2)xCH3, with x ¿ 0, 1, 3, 5 was prepared. Substrates with decreasing stiffness, characterised by the elastic modulus at 37 C, and similar chemical groups were obtained. Firstly, we have investigated whether these minute variations in polymer chemistry lead to differences in fibronectin (FN) adsorption: the same FN density was obtained on every substrate (450 ng cm2 ) but the supramolecular organisation of the protein at the material interface, as obtained with AFM, was different for x ¿ 0 and the other surfaces (x ¿ 1, 3, 5). Consequently, this allows one to use a set of substrates (x ¿ 1, 3, 5) to investigate the effect of substrate stiffness on cell behavior as the unique physical parameter, i.e. after ruling out any influence of the length of the side group on protein conformation. Moreover, the importance of investigating the intermediate layer of proteins at the cellmaterial interface is stressed: the effect of x ¿ 0 and x ¿ 1 on cell behavior cannot be ascribed to the different stiffness of the substrate anymore, since the biological activity of the protein on the material surface was also different. Afterwards, initial cellular interaction was investigated using MC3T3-E1 osteoblasts-like cells and focusing on actin cytoskeleton development, focal adhesion formation and the ability of cells to reorganize the adsorbed FN layer on the different substrates. Image analysis was used to quantify the frequency distribution of the focal plaques, which revealed broader distributions on the stiffer substrates, with formation of larger focal plaques revealing that cells exert higher forces on stiffer substrates.	es_ES
dc.description.sponsorship	The support of the Spanish Ministry of Science and Innovation through project MAT2009-14440-C02-01 is kindly acknowledged. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. This work was supported by funds for research in the field of Regenerative Medicine through the collaboration agreement from the Conselleria de Sanidad (Generalitat Valenciana), and the Instituto de Salud Carlos III.	en_EN
dc.language	Inglés	es_ES
dc.publisher	The Royal Society of Chemistry	es_ES
dc.relation.ispartof	Soft Matter	es_ES
dc.rights	Reserva de todos los derechos	es_ES
dc.subject.classification	FISICA APLICADA	es_ES
dc.subject.classification	TERMODINAMICA APLICADA (UPV)	es_ES
dc.subject.classification	TECNOLOGIA ELECTRONICA	es_ES
dc.title	Subtle variations in polymer chemistry modulates substrate stiffness and fibronectin activity	es_ES
dc.type	Artículo	es_ES
dc.identifier.doi	10.1039/c0sm00074d	es_ES
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//MAT2009-14440-C02-01/ES/Dinamica De Las Proteinas De La Matriz En La Interfase Celula-Material/	es_ES
dc.rights.accessRights	Cerrado	es_ES
dc.contributor.affiliation	Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada	es_ES
dc.contributor.affiliation	Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica	es_ES
dc.contributor.affiliation	Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular	es_ES
dc.contributor.affiliation	Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada	es_ES
dc.description.bibliographicCitation	Brizuela Guerra, N.; González-García, C.; Llopis, V.; Rodriguez-Hernandez, JC.; Moratal, D.; Rico Tortosa, PM.; Salmerón Sánchez, M. (2010). Subtle variations in polymer chemistry modulates substrate stiffness and fibronectin activity. Soft Matter. 6(19):4748-4755. https://doi.org/10.1039/c0sm00074d	es_ES
dc.description.accrualMethod	S	es_ES
dc.relation.publisherversion	https://doi.org/10.1039/c0sm00074d	es_ES
dc.description.upvformatpinicio	4748	es_ES
dc.description.upvformatpfin	4755	es_ES
dc.type.version	info:eu-repo/semantics/publishedVersion	es_ES
dc.description.volume	6	es_ES
dc.description.issue	19	es_ES
dc.relation.pasarela	S\39386	es_ES
dc.contributor.funder	Ministerio de Ciencia e Innovación	es_ES
dc.description.references	Grinnell, F. (1986). Focal adhesion sites and the removal of substratum-bound fibronectin. The Journal of Cell Biology, 103(6), 2697-2706. doi:10.1083/jcb.103.6.2697	es_ES
dc.description.references	Schwarz, U. S., & Bischofs, I. B. (2005). Physical determinants of cell organization in soft media. Medical Engineering & Physics, 27(9), 763-772. doi:10.1016/j.medengphy.2005.04.007	es_ES
dc.description.references	SIPE, J. D. (2002). Tissue Engineering and Reparative Medicine. Annals of the New York Academy of Sciences, 961(1), 1-9. doi:10.1111/j.1749-6632.2002.tb03040.x	es_ES
dc.description.references	Werner, C., Pompe, T., & Salchert, K. (2006). Modulating Extracellular Matrix at Interfaces of Polymeric Materials. Advances in Polymer Science, 63-93. doi:10.1007/12_089	es_ES
dc.description.references	García, A. J. (s. f.). Interfaces to Control Cell-Biomaterial Adhesive Interactions. Advances in Polymer Science, 171-190. doi:10.1007/12_071	es_ES
dc.description.references	Anselme, K., Bigerelle, M., Noel, B., Dufresne, E., Judas, D., Iost, A., & Hardouin, P. (2000). Qualitative and quantitative study of human osteoblast adhesion on materials with various surface roughnesses. Journal of Biomedical Materials Research, 49(2), 155-166. doi:10.1002/(sici)1097-4636(200002)49:2<155::aid-jbm2>3.0.co;2-j	es_ES
dc.description.references	Hynes, R. O. (2002). Integrins. Cell, 110(6), 673-687. doi:10.1016/s0092-8674(02)00971-6	es_ES
dc.description.references	Geiger, B., Bershadsky, A., Pankov, R., & Yamada, K. M. (2001). Transmembrane crosstalk between the extracellular matrix and the cytoskeleton. Nature Reviews Molecular Cell Biology, 2(11), 793-805. doi:10.1038/35099066	es_ES
dc.description.references	Anderson, J. M. (2001). Biological Responses to Materials. Annual Review of Materials Research, 31(1), 81-110. doi:10.1146/annurev.matsci.31.1.81	es_ES
dc.description.references	Wong, J. Y., Leach, J. B., & Brown, X. Q. (2004). Balance of chemistry, topography, and mechanics at the cell–biomaterial interface: Issues and challenges for assessing the role of substrate mechanics on cell response. Surface Science, 570(1-2), 119-133. doi:10.1016/j.susc.2004.06.186	es_ES
dc.description.references	Gray, D. S., Tien, J., & Chen, C. S. (2003). Repositioning of cells by mechanotaxis on surfaces with micropatterned Young’s modulus. Journal of Biomedical Materials Research, 66A(3), 605-614. doi:10.1002/jbm.a.10585	es_ES
dc.description.references	Genes, N. G., Rowley, J. A., Mooney, D. J., & Bonassar, L. J. (2004). Effect of substrate mechanics on chondrocyte adhesion to modified alginate surfaces. Archives of Biochemistry and Biophysics, 422(2), 161-167. doi:10.1016/j.abb.2003.11.023	es_ES
dc.description.references	Schneider, A., Francius, G., Obeid, R., Schwinté, P., Hemmerlé, J., Frisch, B., … Picart, C. (2006). Polyelectrolyte Multilayers with a Tunable Young’s Modulus:  Influence of Film Stiffness on Cell Adhesion. Langmuir, 22(3), 1193-1200. doi:10.1021/la0521802	es_ES
dc.description.references	Ryan, P. L., Foty, R. A., Kohn, J., & Steinberg, M. S. (2001). Tissue spreading on implantable substrates is a competitive outcome of cell-cell vs. cell-substratum adhesivity. Proceedings of the National Academy of Sciences, 98(8), 4323-4327. doi:10.1073/pnas.071615398	es_ES
dc.description.references	Wong, J. Y., Velasco, A., Rajagopalan, P., & Pham, Q. (2003). Directed Movement of Vascular Smooth Muscle Cells on Gradient-Compliant Hydrogels†. Langmuir, 19(5), 1908-1913. doi:10.1021/la026403p	es_ES
dc.description.references	Lo, C.-M., Wang, H.-B., Dembo, M., & Wang, Y. (2000). Cell Movement Is Guided by the Rigidity of the Substrate. Biophysical Journal, 79(1), 144-152. doi:10.1016/s0006-3495(00)76279-5	es_ES
dc.description.references	Bischofs, I. B., & Schwarz, U. S. (2003). Cell organization in soft media due to active mechanosensing. Proceedings of the National Academy of Sciences, 100(16), 9274-9279. doi:10.1073/pnas.1233544100	es_ES
dc.description.references	Schwarz, U. (2007). Soft matters in cell adhesion: rigidity sensing on soft elastic substrates. Soft Matter, 3(3), 263-266. doi:10.1039/b606409d	es_ES
dc.description.references	Choquet, D., Felsenfeld, D. P., & Sheetz, M. P. (1997). Extracellular Matrix Rigidity Causes Strengthening of Integrin–Cytoskeleton Linkages. Cell, 88(1), 39-48. doi:10.1016/s0092-8674(00)81856-5	es_ES
dc.description.references	Vogel, V., & Sheetz, M. (2006). Local force and geometry sensing regulate cell functions. Nature Reviews Molecular Cell Biology, 7(4), 265-275. doi:10.1038/nrm1890	es_ES
dc.description.references	Discher, D. E. (2005). Tissue Cells Feel and Respond to the Stiffness of Their Substrate. Science, 310(5751), 1139-1143. doi:10.1126/science.1116995	es_ES
dc.description.references	Harjanto, D., & Zaman, M. H. (2010). Matrix mechanics and receptor–ligand interactions in cell adhesion. Org. Biomol. Chem., 8(2), 299-304. doi:10.1039/b913064k	es_ES
dc.description.references	Pelham, R. J., & Wang, Y. -l. (1997). Cell locomotion and focal adhesions are regulated by substrate flexibility. Proceedings of the National Academy of Sciences, 94(25), 13661-13665. doi:10.1073/pnas.94.25.13661	es_ES
dc.description.references	Rico, P., Hernández, J. C. R., Moratal, D., Altankov, G., Pradas, M. M., & Salmerón-Sánchez, M. (2009). Substrate-Induced Assembly of Fibronectin into Networks: Influence of Surface Chemistry and Effect on Osteoblast Adhesion. Tissue Engineering Part A, 15(11), 3271-3281. doi:10.1089/ten.tea.2009.0141	es_ES
dc.description.references	Otsu, N. (1979). A Threshold Selection Method from Gray-Level Histograms. IEEE Transactions on Systems, Man, and Cybernetics, 9(1), 62-66. doi:10.1109/tsmc.1979.4310076	es_ES
dc.description.references	Altankov, G., Thom, V., Groth, T., Jankova, K., Jonsson, G., & Ulbricht, M. (2000). Modulating the biocompatibility of polymer surfaces with poly(ethylene glycol): Effect of fibronectin. Journal of Biomedical Materials Research, 52(1), 219-230. doi:10.1002/1097-4636(200010)52:1<219::aid-jbm28>3.0.co;2-f	es_ES
dc.description.references	García, A. (1999). Integrin–fibronectin interactions at the cell-material interface: initial integrin binding and signaling. Biomaterials, 20(23-24), 2427-2433. doi:10.1016/s0142-9612(99)00170-2	es_ES
dc.description.references	Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006). Matrix Elasticity Directs Stem Cell Lineage Specification. Cell, 126(4), 677-689. doi:10.1016/j.cell.2006.06.044	es_ES
dc.description.references	Keselowsky, B. G., Collard, D. M., & Garcia, A. J. (2005). Integrin binding specificity regulates biomaterial surface chemistry effects on cell differentiation. Proceedings of the National Academy of Sciences, 102(17), 5953-5957. doi:10.1073/pnas.0407356102	es_ES
dc.description.references	Barrias, C. C., Martins, M. C. L., Almeida-Porada, G., Barbosa, M. A., & Granja, P. L. (2009). The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers. Biomaterials, 30(3), 307-316. doi:10.1016/j.biomaterials.2008.09.048	es_ES
dc.description.references	Khatiwala, C. B., Peyton, S. R., & Putnam, A. J. (2006). Intrinsic mechanical properties of the extracellular matrix affect the behavior of pre-osteoblastic MC3T3-E1 cells. American Journal of Physiology-Cell Physiology, 290(6), C1640-C1650. doi:10.1152/ajpcell.00455.2005	es_ES
dc.description.references	Kong, H. J., Polte, T. R., Alsberg, E., & Mooney, D. J. (2005). FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness. Proceedings of the National Academy of Sciences, 102(12), 4300-4305. doi:10.1073/pnas.0405873102	es_ES
dc.description.references	Keselowsky, B. G., Collard, D. M., & Garcı́a, A. J. (2004). Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding. Biomaterials, 25(28), 5947-5954. doi:10.1016/j.biomaterials.2004.01.062	es_ES
dc.description.references	Keselowsky, B. G., Collard, D. M., & García, A. J. (2003). Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion. Journal of Biomedical Materials Research Part A, 66A(2), 247-259. doi:10.1002/jbm.a.10537	es_ES
dc.description.references	Gugutkov, D., González-García, C., Rodríguez Hernández, J. C., Altankov, G., & Salmerón-Sánchez, M. (2009). Biological Activity of the Substrate-Induced Fibronectin Network: Insight into the Third Dimension through Electrospun Fibers. Langmuir, 25(18), 10893-10900. doi:10.1021/la9012203	es_ES
dc.description.references	Gugutkov, D., Altankov, G., Rodríguez Hernández, J. C., Monleón Pradas, M., & Salmerón Sánchez, M. (2010). Fibronectin activity on substrates with controlled OH density. Journal of Biomedical Materials Research Part A, 92A(1), 322-331. doi:10.1002/jbm.a.32374	es_ES
dc.description.references	Bae, Y. H., Johnson, P. A., Florek, C. A., Kohn, J., & Moghe, P. V. (2006). Minute changes in composition of polymer substrates produce amplified differences in cell adhesion and motility via optimal ligand conditioning. Acta Biomaterialia, 2(5), 473-482. doi:10.1016/j.actbio.2006.04.001	es_ES
dc.description.references	Nicolas, A., Geiger, B., & Safran, S. A. (2004). Cell mechanosensitivity controls the anisotropy of focal adhesions. Proceedings of the National Academy of Sciences, 101(34), 12520-12525. doi:10.1073/pnas.0403539101	es_ES
dc.description.references	Balaban, N. Q., Schwarz, U. S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., … Geiger, B. (2001). Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates. Nature Cell Biology, 3(5), 466-472. doi:10.1038/35074532	es_ES
dc.relation.references	10.1083/jcb.103.6.2697	es_ES
dc.relation.references	10.1016/j.medengphy.2005.04.007	es_ES
dc.relation.references	10.1111/j.1749-6632.2002.tb03040.x	es_ES
dc.relation.references	10.1007/12_089	es_ES
dc.relation.references	10.1007/12_071	es_ES
dc.relation.references	10.1002/(SICI)1097-4636(200002)49:2<155::AID-JBM2>3.0.CO;2-J	es_ES
dc.relation.references	10.1016/S0092-8674(02)00971-6	es_ES
dc.relation.references	10.1038/35099066	es_ES
dc.relation.references	10.1146/annurev.matsci.31.1.81	es_ES
dc.relation.references	10.1016/j.susc.2004.06.186	es_ES
dc.relation.references	10.1002/jbm.a.10585	es_ES
dc.relation.references	10.1016/j.abb.2003.11.023	es_ES
dc.relation.references	10.1021/la0521802	es_ES
dc.relation.references	10.1073/pnas.071615398	es_ES
dc.relation.references	10.1021/la026403p	es_ES
dc.relation.references	10.1016/S0006-3495(00)76279-5	es_ES
dc.relation.references	10.1073/pnas.1233544100	es_ES
dc.relation.references	10.1039/B606409D	es_ES
dc.relation.references	10.1016/S0092-8674(00)81856-5	es_ES
dc.relation.references	10.1038/nrm1890	es_ES
dc.relation.references	10.1126/science.1116995	es_ES
dc.relation.references	10.1039/B913064K	es_ES
dc.relation.references	10.1073/pnas.94.25.13661	es_ES
dc.relation.references	10.1089/ten.tea.2009.0141	es_ES
dc.relation.references	10.1109/TSMC.1979.4310076	es_ES
dc.relation.references	10.1002/1097-4636(200010)52:1<219::AID-JBM28>3.0.CO;2-F	es_ES
dc.relation.references	10.1016/S0142-9612(99)00170-2	es_ES
dc.relation.references	10.1016/j.cell.2006.06.044	es_ES
dc.relation.references	10.1073/pnas.0407356102	es_ES
dc.relation.references	10.1016/j.biomaterials.2008.09.048	es_ES
dc.relation.references	10.1152/ajpcell.00455.2005	es_ES
dc.relation.references	10.1073/pnas.0405873102	es_ES
dc.relation.references	10.1016/j.biomaterials.2004.01.062	es_ES
dc.relation.references	10.1002/jbm.a.10537	es_ES
dc.relation.references	10.1021/la9012203	es_ES
dc.relation.references	10.1002/jbm.a.32374	es_ES
dc.relation.references	10.1016/j.actbio.2006.04.001	es_ES
dc.relation.references	10.1073/pnas.0403539101	es_ES
dc.relation.references	10.1038/35074532	es_ES



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