Multicolor plate reader fluorescence calibration
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| dc.contributor.author | Beal, Jacob
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es_ES |
| dc.contributor.author | Telmer, Cheryl A.
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es_ES |
| dc.contributor.author | Vignoni, Alejandro
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es_ES |
| dc.contributor.author | Boada-Acosta, Yadira Fernanda
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es_ES |
| dc.contributor.author | Baldwin, Geoff S.
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es_ES |
| dc.contributor.author | Hallett, Liam
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es_ES |
| dc.contributor.author | Lee, Taeyang
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es_ES |
| dc.contributor.author | Selvarajah, Vinoo
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es_ES |
| dc.contributor.author | Billerbeck, Sonja
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es_ES |
| dc.contributor.author | Brown, Bradley
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es_ES |
| dc.contributor.author | Cai, Guo-nan
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es_ES |
| dc.contributor.author | Cai, Liang
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es_ES |
| dc.contributor.author | Eisenstein, Edward
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es_ES |
| dc.contributor.author | Kiga, Daisuke
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es_ES |
| dc.contributor.author | Ross, David
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es_ES |
| dc.date.accessioned | 2024-04-24T18:06:18Z | |
| dc.date.available | 2024-04-24T18:06:18Z | |
| dc.date.issued | 2022-02-02 | es_ES |
| dc.identifier.uri | http://hdl.handle.net/10251/203726 | |
| dc.description | This is a pre-copyedited, author-produced version of an article accepted for publication in Synthetic Biology following peer review. The version of record Jacob Beal, Cheryl A Telmer, Alejandro Vignoni, Yadira Boada, Geoff S Baldwin, Liam Hallett, Taeyang Lee, Vinoo Selvarajah, Sonja Billerbeck, Bradley Brown, Guo-nan Cai, Liang Cai, Edward Eisenstein, Daisuke Kiga, David Ross, Nina Alperovich, Noah Sprent, Jaclyn Thompson, Eric M Young, Drew Endy, Traci Haddock-Angelli, Multicolor plate reader fluorescence calibration, Synthetic Biology, Volume 7, Issue 1, 2022, ysac010, https://doi.org/10.1093/synbio/ysac010, is available online at: https://doi.org/10.1093/synbio/ysac010. | es_ES |
| dc.description.abstract | [EN] Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously established a robust serial dilution protocol for calibration of plate reader measurements of absorbance to estimated bacterial cell count and for green fluorescence from proteins expressed in bacterial cells to molecules of equivalent fluorescein. We now extend these protocols to calibration of red fluorescence to the sulforhodamine-101 fluorescent dye and blue fluorescence to Cascade Blue. Evaluating calibration efficacy via an interlaboratory study, we find that these calibrants do indeed provide comparable precision to the prior calibrants and that they enable effective cross-laboratory comparison of measurements of red and blue fluorescence from proteins expressed in bacterial cells. | es_ES |
| dc.description.sponsorship | This work was supported in part by the following funding sources: J.B. was supported by Air Force Research Laboratory (AFRL) and DARPA contract FA8750-17-C-0184. N.S. was supported by funding from the BBSRC under award BB/M011178/1. G.B., L.H., and T.L. were supported by the EPSRC under award EP/R034915/1 and EP/S022856/1. G.C. and L.C. were supported by funds from YF Capital and the National Top Talent Undergraduate Training Program, China. A.V. and Y.B. were funded by Grant MINECO/AEI, EU DPI2017-82896-C2-1-R and MCIN/AEI/10.13039/501100011033 grant number PID2020117271RB-C21. Y.B. was supported by Secretaria de Educaci ' on Superior, Ciencia, Tecnologia e Innovacion-Ecuador (Scholarship Convocatoria Abierta 2011). D.K. was supported by JST, CREST Grant Number JPMJCR21N4, Japan. E.Y. was supported by the National Science Foundation under Grant No. 1939860. J.T. was supported by the Office of the Director of National Intelligence (ODNI), Intelligence Advanced Research Projects Activity (IARPA) under Finding Engineering Linked Indicators (FELIX) program contract N66001-18-C-4507. This document does not contain technology or technical data controlled under either the U.S. International Traffic in Arms Regulations or the U.S. Export Administration Regulations. Views, opinions, and/or findings expressed are those of the author(s) and should not be interpreted as representing the official views or policies of the Department of Defense or the U.S. Government. | es_ES |
| dc.language | Inglés | es_ES |
| dc.publisher | Oxford University Press | es_ES |
| dc.relation.ispartof | Synthetic Biology | es_ES |
| dc.rights | Reconocimiento - No comercial (by-nc) | es_ES |
| dc.subject | Calibration | es_ES |
| dc.subject | Units | es_ES |
| dc.subject | Fluorescence | es_ES |
| dc.subject | Cell count | es_ES |
| dc.subject.classification | INGENIERIA DE SISTEMAS Y AUTOMATICA | es_ES |
| dc.title | Multicolor plate reader fluorescence calibration | es_ES |
| dc.type | Artículo | es_ES |
| dc.identifier.doi | 10.1093/synbio/ysac010 | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI//DPI2017-82896-C2-1-R-AR//DISEÑO, CARACTERIZACION Y AJUSTE OPTIMO DE BIOCIRCUITOS SINTETICOS PARA BIOPRODUCCION CON CONTROL DE CARGA METABOLICA/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/AGENCIA ESTATAL DE INVESTIGACION//PID2020-117271RB-C21//REGULACIÓN DINÁMICA MULTI-ESCALA EN INGENIERÍA METABÓLICA: CONTROL REALIMENTADO Y CICLO DBTL AUTOMATIZADO/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/NSF//1939860/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/EPSRC//EP%2FR034915%2F1/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/EPSRC//EP%2FS022856%2F1/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/JST//JPMJCR21N4/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/BBSRC//BB%2FM011178%2F1/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/DARPA//FA8750-17-C-0184/ | es_ES |
| dc.relation.projectID | info:eu-repo/grantAgreement/IARPA//N66001-18-C-4507//Finding Engineering Linked Indicators (FELIX)/ | es_ES |
| dc.rights.accessRights | Abierto | es_ES |
| dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny | es_ES |
| dc.description.bibliographicCitation | Beal, J.; Telmer, CA.; Vignoni, A.; Boada-Acosta, YF.; Baldwin, GS.; Hallett, L.; Lee, T.... (2022). Multicolor plate reader fluorescence calibration. Synthetic Biology. 7(1):1-9. https://doi.org/10.1093/synbio/ysac010 | es_ES |
| dc.description.accrualMethod | S | es_ES |
| dc.relation.publisherversion | https://doi.org/10.1093/synbio/ysac010 | es_ES |
| dc.description.upvformatpinicio | 1 | es_ES |
| dc.description.upvformatpfin | 9 | es_ES |
| dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
| dc.description.volume | 7 | es_ES |
| dc.description.issue | 1 | es_ES |
| dc.identifier.eissn | 2397-7000 | es_ES |
| dc.identifier.pmid | 35949424 | es_ES |
| dc.identifier.pmcid | PMC9357555 | es_ES |
| dc.relation.pasarela | S\486332 | es_ES |
| dc.contributor.funder | Yunfeng Capital | es_ES |
| dc.contributor.funder | Air Force Research Laboratory | es_ES |
| dc.contributor.funder | AGENCIA ESTATAL DE INVESTIGACION | es_ES |
| dc.contributor.funder | National Science Foundation, EEUU | es_ES |
| dc.contributor.funder | Japan Science and Technology Agency | es_ES |
| dc.contributor.funder | Defense Advanced Research Projects Agency | es_ES |
| dc.contributor.funder | Office of the Director of National Intelligence | es_ES |
| dc.contributor.funder | Intelligence Advanced Research Projects Activity | es_ES |
| dc.contributor.funder | Engineering and Physical Sciences Research Council, Reino Unido | es_ES |
| dc.contributor.funder | Biotechnology and Biological Sciences Research Council, Reino Unido | es_ES |
| dc.contributor.funder | Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador | es_ES |
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