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Specific sets of geranylgeranyl diphosphate synthases and phytoene synthases control the production of carotenoids and ABA in different tomato tissues

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Specific sets of geranylgeranyl diphosphate synthases and phytoene synthases control the production of carotenoids and ABA in different tomato tissues

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dc.contributor.author Burbano-Erazo, Esteban es_ES
dc.contributor.author Ezquerro-Urzanqui, Miguel es_ES
dc.contributor.author Sánchez-Bel, Paloma es_ES
dc.contributor.author Rodriguez-Concepción, Manuel es_ES
dc.date.accessioned 2025-02-25T19:08:31Z
dc.date.available 2025-02-25T19:08:31Z
dc.date.issued 2025-01 es_ES
dc.identifier.issn 0031-9317 es_ES
dc.identifier.uri http://hdl.handle.net/10251/214818
dc.description.abstract [EN] Plant carotenoids are plastid-synthesized isoprenoids with roles as photoprotectants, pigments, and precursors of bioactive molecules such as the hormone abscisic acid (ABA). The first step of the carotenoid biosynthesis pathway is the production of phytoene from geranylgeranyl diphosphate (GGPP), catalyzed by phytoene synthase (PSY). GGPP produced by plastidial GGPP synthases (GGPPS) is channeled to the carotenoid pathway by direct interaction of GGPPS and PSY enzymes. Three plastid-localized GGPPS isoforms (referred to as SlG1-3) and three PSY enzymes (PSY1-3) are present in tomato (Solanum lycopersicum). Our previous work showed that SlG1 and PSY3 function together in the roots, whereas the rest of the isoforms are required in aerial tissues. Here we generated and analyzed combinations of double mutants lacking PSY1 or PSY2 and SlG2 or SlG3 to investigate the contribution of specific GGPPS and PSY pairs to the production of carotenoids and ABA in different tissues of the tomato plant. Despite that the loss of individual enzymes was found to trigger compensatory mechanisms that complicate interpretation of the results, the results confirm a major role for SlG3 in providing GGPP to PSY2 for housekeeping carotenoid biosynthesis in leaves, whereas SlG2 and PSY1 become most relevant when a more active production is required in flowers and breaker fruits, i.e., at the onset of ripening. We could also confirm that ABA production in the fruit pericarp is more dependent on PSY1 activity than on total carotenoid levels and that fruit size correlates with ABA levels accumulated in ripe rather than breaker fruits. es_ES
dc.description.sponsorship Agencia Estatal de Investigación, Grant/Award Numbers: BES-2017-080652, PCI2021-121941, PID2020115810GB-I00, PID2023-149584NB-I00, RED2022-134577-T; Generalitat Valenciana, Grant/Award Numbers: AGROALNEXT/2022/067, PROMETEU/2021/056; Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS), Grant/Award Number: MINCIENCIAS885/2020 es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Physiologia Plantarum es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Geranylgeranyl diphosphate synthases es_ES
dc.subject Carotenoids es_ES
dc.subject ABA es_ES
dc.title Specific sets of geranylgeranyl diphosphate synthases and phytoene synthases control the production of carotenoids and ABA in different tomato tissues es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/ppl.70052 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2021-121940/ES/HANDY TOOLS FOR SUSTAINABLE IRRIGATION MANAGEMENT IN MEDITERRANEAN CROPS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115810GB-I00/ES/MECANISMOS MOLECULARES DE LA BIOGENESIS DE CROMOPLASTOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2021%2F056/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149584NB-I00/ES/REDES DE INTERACCION DE PROTEINAS QUE REGULAN LA BIOSINTESIS DE CAROTENOIDES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//RED2022-134577-T/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//BES-2017-080652/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGROALNEXT%2F2022%2F067/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//MINCIENCIAS885%2F2020/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Burbano-Erazo, E.; Ezquerro-Urzanqui, M.; Sánchez-Bel, P.; Rodriguez-Concepción, M. (2025). Specific sets of geranylgeranyl diphosphate synthases and phytoene synthases control the production of carotenoids and ABA in different tomato tissues. Physiologia Plantarum. 177(1). https://doi.org/10.1111/ppl.70052 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/ppl.70052 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 177 es_ES
dc.description.issue 1 es_ES
dc.relation.pasarela S\540458 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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